Иммунные исследования
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Immunoinformatics Prediction of Epitope Based Peptide Vaccine against Clostridium perfringens Fructose Bisphosphate Aldolase Protein

Mustafa Elhag, Tebyan Ameer Abdelhameed, Moaaz Mohammed Saadaldin, Abdelrahman Hamza Abdelmoneim, Taha Salah Taha, Israa Abdulrahman Mohammed, Mohammed A.Hassan

Clostridium perfringens is a member of the gastrointestinal tract (GIT) microbial community for both diseased and healthy humans and animals. Also, this bacterium is responsible for around 5%–15% of all circumstances of antibiotic-associated diarrhea, which develops in 5%–40% of all patients receiving antibiotic therapy. In addition, it causes enteritis necroticans; an often-fatal human disease. C. perfringens is clear and defined the underlying factors responsible for specific aspects of pathology remains uncertain. This study predicts an effective epitope-based vaccine against fructose 1,6-biphosphate aldolase (FBA) enzyme of Clostridium perfringens using immunoinformatics tools. The sequences were retrieved from NCBI and several prediction tests were conducted to analyze possible epitopes for B-cell, T-cell MHC class I and II. Tertiary structure of the most promising epitopes was obtained. 48 epitopes showed high binding affinity for B-cells, while five epitopes showed high binding affinity for MHC I and MHC II. The results were promising to formulate a vaccine with more than 98% population coverage. We hope that these promising epitopes serves as a preventive measure for the disease in the future and recommend in vivo and in vitro studies.