Журнал хроматографии и методов разделения
Открытый доступ

Абстрактный

A Rapid and Effective Method for Simultaneous Determination of Residual Sulfonamides and Sarafloxacin in Pork and Chicken Muscle by High Performance Liquid Chromatography with Accelerated Solvent Extraction–Solid Phase Extraction Cleanup

Hanwen Sun, Yun Hao and Xingqiang Wu

A rapid and effective high performance liquid chromatographic (HPLC) method with accelerated solvent extraction (ASE)-solid phase extraction cleanup was developed for the determination of sulfonamide and sarafloxacin residues in pork and chicken muscle. Four residues were extracted with acetic acid-acetonitrile at 70°C under 10.3 MPa pressure and 5 min static times with 2 static cycles. The standard curves for the determination of four residues have good linearity (r>0.999). The method limits of quantification (LOQs) were 4-12 μg.kg-1. Intra- and inter-day precisions (RSDs) were 0.3-1.5% and 0.7-1.8%, respectively. Their average recoveries with four spiked levels ranged from 83% to 108% with the RSD of 0.3-1.5%. This method provides an effective extraction procedure and high sensitivity, and can be applied for the determination of 4 residual drugs in pork and chicken muscle at lower than their MRL levels.