Фармацевтическая аналитическая химия: открытый доступ

Фармацевтическая аналитическая химия: открытый доступ
Открытый доступ

ISSN: 2471-2698

Абстрактный

Применение эксперимента типа 19F-водной логарифмии для скрининга фторированных соединений на основе ЯМР

Kazuo Furihata, Hiroaki Utsumi, Toshiyo Kato, Chiseko Sakuma and Mitsuru Tashiro

The WaterLOGSY type experiment with 19F detection was applied to observe the interaction between a fluorinated compound and a macromolecule. The proposed experiment, which was developed based on the 19F{1H} saturation transfer difference experiment, was carried out using the conventional spectrometer equipped with a single high band amplifier and a H/F/C-double tuned probe. The selective 19F detection is advantageous in screening the fluorinated compounds, considering that 19F is a sensitive nucleus in NMR spectroscopy. The effective approach to discriminate binding of the fluorinated compounds to proteins with 19F detection was demonstrated using the complex of diflunisal and human serum albumin.

Отказ от ответственности: Этот тезис был переведен с использованием инструментов искусственного интеллекта и еще не прошел рецензирование или проверку.
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