Журнал хроматографии и методов разделения
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ISSN: 2157-7064
ISSN: 2157-7064
Sushmita I. Hiremath, Mahesh. S. Palled* , Rajesh K Joshi, Mahendra Kumar Chouhan, Shailendra S. Suryawanshi
Objective: The objective of present work is to develop and validate HPTLC method for simultaneous estimation of Curcumin and Azadirachtin in marketed formulation
Materials and methods: HPTLC method was developed using a solvent system Toluene: Ethyl acetate: Ammonia: Formic acid (4:3:2.5:0.5 v/v/v/v) using a stationary phase Silica Gel 60 F254 and the saturation time is 15 min. The developed method was standardized in terms of validation parameters such as specificity, linear range, precision, robustness, ruggedness and reproducibility as per ICH guidelines. Newly developed and validated method was successfully applied for estimation of Curcumin and Azadirachtin in marketed formulation.
Results: The linearity range for the both Curcumin and Azadirachtin was found to be 1.5-15 μl/spot. The limit of detection found to be 0.383213 μl/spot for the Curcumin and 0.46572 μl/spot for Azadirachtin. The limit of quantification is found to be 1.16125 μl/spot for the Curcumin and 1.411272 μl/spot for Azadirachtin. Recovery of Azadirachtin in marketed formulation was observed in the range of 91%-109% and recovery of Curcumin in marketed formulation was observed in the range of 91%-105%. All the precision and repeatability results were within acceptance range less than 2%. Assay of Curcumin and Azadirachtin was found to be 92.95% and 91.79% respectively. The Rf value of Curcumin is found to be 0.5 ± 0.03 and the Rf value of Azadirachtin is found to be 0.57 ± 0.04.
Conclusion: The method was found to be simple, accurate, environment friendly, reproducible and can be used for routine estimation analysis of Curcumin and Azadirachtin in marketed formulation.