Журнал хроматографии и методов разделения
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ISSN: 2157-7064
ISSN: 2157-7064
Pradip N Bhoya, Emanual M Patelia and Gautambhai
A new simple, precise, accurate and selective TLC-densitometry method has been developed for simultaneous determination of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form. Chromatographic separation was performed on aluminum plate precoated with silica gel 60F254, using chloroform: ethanol: glacial acetic acid 5:1.5:0.2 (v/v) as mobile phase. Detection was carried out densitometrically at 225 nm. The R value of bisoprolol fumarate and hydrochlorothiazide were 0.62 and 0.40, respectively. The reliability of the method was assessed by evaluation of linearity which was found to be 200–1200 ng/spot for bisoprolol fumarate and 100-800 ng/spot for hydrochlorothiazide. Accuracy of the method was accessed by % recovery and found to be 100.02 ± 1.14% for bisoprolol fumarate and 99.91 ± 0.96% for hydrochlorothiazide. The method can be used for routine analysis of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form.