Журнал клинических и экспериментальных дерматологических исследований
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New Bioplastic Material Based on Hyaluronic Acid Hydrocolloid

Zinoviev EV, Rakhmatullin RR and Almazov IA

Relief of the typical pathological processes and the early activation of regenerative mechanisms are the conditions for the successful wound healing. Reparative potential of epithelial tissues is realized at a balanced migration and proliferation of their cellular elements, synthesis of fibrous proteins and extracellular matrix components, epithelisation. An in-depth assessment was made of the structure of the developed bioplastic material based on hyaluronic acid hydrocolloid (G-Derm) and of its properties, positive in terms of optimizing the reparative mechanisms. Scanning probe microscopy and ultrastructure evaluation of bioplastic material were performed. For investigating the visualized globular structures, homogeneous samples of the material length 101.5 ± 11.2 nm, width 110.3 ± 10.7 nm, height 23.4 ± 3.4 nm were taken. The space between globular formations was 127.2 ± 21.3 nm. It was found that the roughness coefficient of the surface relief (Rq) was 8.7 ± 0.5 nm. The method of fixing the contact angle of water was used to evaluate hydrophilic/hydrophobic properties of biomaterial. Value of the angle was 83°; adhesion coefficient was 99.88 mN/ m2, which characterizes the surface of biomaterial as moderately hydrophilic. Mesenchymal stromal stem cells were cultured on a substrate of bioplastic material based on hyaluronic acid hydrocolloid. In the course of ultrastructural study cell migration into the material was recorded, presence between the interwoven fibrillar fibres of cell layers of an oblong shape 3.7 ± 0.5 mcm wide, which reflects their cooperation with the surface of the developed polymer suitable for the use as a growth substrate in case of biotechnological replacement of tissue defects.