Журнал прикладной фармации
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ISSN: 1920-4159
ISSN: 1920-4159
Irum Latif, Riffat Latif, Iftikhar Khan, Maida Minahil Mushtaq, Muhammad Zubair, Qurat-ul Ain, Rizwan Khalid
Folklore herbal practioners of Cholistan desert claim Corchorus depressus Linn. (Tiliaceae) treat diabetes pain, fever, gonorrhea, treachery troubles, general weakness and sexual dysfunction. The aim of this study was to isolate α-glucosidase and anti-urease inhibitors and to check the α-glucosidase and anti-urease seasonal in three different time periods. In vitro α-glucosidase and anti-urease assays were carried out and in vivo anti-diabetic activity of Corchorus depressus Linn. Was studied on alloxan induced diabetic rats to show its traditional use. The fractions CD-13, CD-14, CD-15 and CD-20 showed 4.31 ± 0.07, 6.32 ± 0.08, 15.37 ± 0.13 and 12.42 ± 0.13 μg/mL with respect to Acarbose with IC50 37.38 ± 0.12 μM. The fraction CD-M7 and CD-J9 extracts were found active against anti-urease activity with IC50 1.63 ± 0.08 and 2.42 ± 0.07 μg/mL, respectively, as compared to thiourea with IC50 21.46 ± 0.13 μM. CDM7 and CD-J9 extracts were found active with IC50 1.63 ± 0.08 and 2.42 ± 0.07 μg/mL, respectively, as compared to thiourea with IC50 21.46 ± 0.13 μM. The most potent fraction CD-14 CDB was subjected to GC-MS analysis which resulted in isolation of metabolites as given in Table 1. Our results validate the traditional use of Corchorus depressus for traditional therapeutic potential in treating diabetes and ulcer diseases. So it was concluded from the following discussion that non-polar CD fractions (upto 20% EtOAc/Pet. ether) showed potent IC50 values for α-glucosidase inhibition but CD polar (water extracts) showed remarkable IC50 values for anti-urease inhibition.