Журнал хроматографии и методов разделения
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ISSN: 2157-7064
ISSN: 2157-7064
Ibrahim F, Sharaf El-Din M and Heba Abd El-Aziz
A high performance liquid chromatographic method characterized by its rapidness and sensitivity was developed and validated for quantitation of Cilostazol (CIL) and Telmisartan (TEL) in raw material, their synthetic mixture using isocratic technique and monolithic C8 column (3 mm × 4.6 mm i.d., 2 μm pore size highly porous). The mobile phase composed of acetonitrile:0.03 M dihydrogen phosphate buffer (40:60, v/v) at pH 4.5. Quantification was achieved through UV detection at 257 nm using flow rate of 1 mL/min, and Dipyridamole (DIP) was used as internal standard. DIP, CIL, and TEL, retention times were 2.2, 3.9 and 5.1 min. respectively. Peak area ratios of each drug to Dipyridamol internal standard were plotted against concentration of each drug and linear relations were obtained in the range of 0.5-15 μg/mL for CIL 0.25-20 μg /mL for TEL. The method was successfully utilized for the assay of CIL and TEL synthetic mixture. Stability-indicating assay methods (SIAM) were mentioned for separation of CIL in presence of its degradation products. Cilostazol was subjected to acid and alkali hydrolysis, oxidation and photochemical degradation. It was stable under acidic, basic and ultraviolet degradation conditions, but undergoes oxidative degradation, therefore the drug was separated from its oxidative degradation product using our proposed high performance liquid chromatographic and derivative ultraviolet
spectrophotometric
methods. The first derivative method (D1) depend on measuring the amplitude values at 227 and 257 nm for Cilostazol and oxidative degradate, respectively. From calibration plots, linearity was obtained in the range of 1-35 μg/mL for Cilostazol and 2-50 μg/mL for oxidative degradate. Chromatographic separation of Cilostazol from its oxidative degradate was proceeded using the same mobile phase at pH 3.3. All methods were validated statistically as per International Conference on Harmonization (ICH) recommendations for the studied drugs and Cilostazol oxidative degradate in the concentration range of the suggested methods se ion-pair liquid chromatography was described.