Журнал хроматографии и методов разделения
Открытый доступ

Абстрактный

Simultaneous Monitoring of Multiple Transitions in Mass Spectrometric Analysis Improves Limit of Detection for Low Abundance Substances in Complex Biological Samples

Dmitriy Shin, Eduard Rogatsky and Alexander V Stoyanov

In mass-spectrometry, simultaneous monitoring of several transitions enables increased assay specificity, but also can be used as a tool to improve the lower limit of quantification. In this report, we propose a new approach to low abundant analyte detection in complex samples. The method consists of collecting two and potentially more, different mass spectrometry transitions with further analysis of a product function. For example, C-peptide detection was evaluated with two transitions monitored (1007.7-147.2 and 1007.7-927.8). Further extension of the method is theoretically analyzed and its potential limitations are discussed.