Журнал генетических синдромов и генной терапии
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Stem Cells Transplantation in Myocardial Tissue Induces Pro-arrhythmic Effects and Promotes Reperfusion. Comparison between Intramyocardial and Intravenous Approach

Pennella S, Reggiani Bonetti L, Migaldi M, Farinetti A, Manenti A, Lonardi R, Giuliani E, Panini F and Mattioli AV

Background: The aim of study was to evaluate the histologic changes and pro-arrhythmic features of intramyocardial stem cells transplantation after myocardial ischemia.

Methods: In 21 New Zealand rabbits an ischemia/reperfusion injury was induced by temporary ligation of anterior coronary artery during cardiac surgical procedure. Mesenchymal bone marrow stem cells (BMSCs) were isolated, cultured and re-suspended for injection. BMSCs were injected at the peri-infarcted area and side effects were evaluated and correlated with histological changes. Ventricular premature contractions (VPCs) were recorded during surgery and after 7 and 21 days.

Results: Frequent VPCs were observed before and after cells administration. The 7th day after the surgery, the episodes of VPC are more frequent in the group that received i.m. BMSCs and in the group that received i.m. administration of saline, compared to animals treated with BMSCs i.v. (135 ± 23; 52 ± 12; and 38 ± 9, respectively, P <0.01 within groups), while, after 21 days, the number of VPC was higher in the group treated with BMSCs by i.m. compared to the group treated with BMSC i.v. compared to the group treated with saline i.m. (100 ± 35 vs. 58 ± 22;and 24 ± 12 i.m saline, P <0.001 within groups).