Эндокринология и метаболический синдром

Эндокринология и метаболический синдром
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ISSN: 2161-1017


Stress during Lactation Affects Fatty Acid Amide Hydrolase Protein Expression in Adipose Tissue and Liver of Adult Mice

Valeska AC, Carina AV, Paula P, Ana MR and Miguel NL

CIntroduction: Early stress alters the endo-cannabinoid system of tissues involved in energy homeostasis, leading to long term consequences associated to hormonal and metabolic disruptions associated to overweight/obesity and insulin resistance. We have previously reported a decreased Anandamide (AEA)-hydrolysing activity in liver and epididymal fat of adult mice previously subjected to stress during lactation, a result that may indicate reduced activity of the enzyme fatty acid amide hydrolase present in both tissues (FAAH; able to hydrolyse the endocannabinoid AEA).

Objective: Since at present it is unknown whether early stress affects FAAH gene and/or protein expression in liver and adipose tissue during adulthood, we have performed studies with the aim of evaluating these parameters.

Methods: Male mice pups were subjected to a soft nociceptive stress during the first 21 days of life (whole lactation period) with a subcutaneous injection of saline solution in the back, and sacrificed when adults (130 days old) to extract liver and epididymal fat.

Results: Data obtained from real time PCR demonstrate that stress during lactation do not affect FAAH mRNA expression either in liver or epididymal adipose tissue of adult animals; however, Western Blot analyses indicates that FAAH protein amount was decreased by 35 and 65% in liver and epididymal fat, respectively.

Conclusion: Present results demonstrate that nociceptive stress during lactation leads to decreased FAAH protein expression in liver and epididymal fat of adult mice, which may be associated to the low AEA- hydrolysing activity previously reported by us in both tissues. Low levels and activity of FAAH should result in increased availability of AEA, leading to a sustained activity of type 1 cannabinoid receptors associated to undesirable cellular and physiological consequences