Журнал медицинских методов диагностики
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The Advanced Bright-field Light Optical Polarization Microscopy: Novel Coupling Method for Detection of Micro Vesicles

Berezin AE, Mokhnach RE

Micro vesicles (MVs) are secreted as a component of secretome of various cells including stem cells, progenitors/ precursors and mature cells with different origin. MV are found to be a biological markers reflecting pathophysiological stages of disease, i.e. cardiovascular (CV) and metabolic diseases, autoimmune diseases, malignancy, infections and eclampsia. The fundamental effect of MV affects cell-to-cell cooperation, regulation of immune interrelations and transferring of several molecules. Therefore, there is a large body of evidence that confirmed the fact of number and phenotypes of MVs may be as a target for personal medical care as well as cargo for drug to the target cells. In this context, laboratory methods regarding purification of MV samples, determination of MVs, identification of origin of them and measure their concentration are fairly promising. The short communication is devoted to the consideration of advantages of novel bright-field light optical polarization microscopy, which is posed as an alternative quantified free-labeled measurement of main characteristics of MVs.