ISSN: 2471-9315
Цзиньмей Линь, Цзинна Лю, Цзяофэнь Линь, Цзяфу Хуан, Чжичао Линь, Бо Ленг, Юй Сюэ, И Су, Лиюнь Яо, Сюмин Ли, Юаньхун Чжуан и Ютянь Пань
Four structural substances were extracted from P. eryngii, including PEE-1, PEE-2, PEE-3 and PEE-4. The chemical analysis was conducted to measure the contents of polysaccharide and protein in PEE-1, results found that they were 10.78% and 26.66% respectively. PEE-2, PEE-3 and PEE-4 were respectively identified as β-glucan, chitosan and D-glucosamine hydrochloride by FT-IR, GC, HPLC and HPLC-MS. The results showed that PEE-1, PEE-2 and PEE-3 could inhibit the activity of α-glycosidase from the rabbit isolated small intestine, while the PEE-4 had no inhibitory effect. At the same concentration, the PEE-1 showed more significantly inhibitory effects on α- glycosidase activity than Glucobay (P<0.05). The hypoglycemic effect of PEE-1 had significant differences in the blood glucose between each group and model control group (P<0.05). In glucose tolerance test, the other groups had significant differences compared with the model control group (P<0.05). In addition, high dose of PEE-1 normal group had no significant effect on the fasting blood glucose level with normal control group (P>0.05). Results showed that PEE-1 had the hypoglycaemic function in hyperglycaemia animals, and it could be applied in the research and production of hypoglycaemic health food.