Журнал протеомики и биоинформатики
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2'-5'-Linked Triadenylates Act as Protein Kinase Activity Modulators

Skorobogatov OY, Kukharenko AP, Kozlov OV, Dubey IY and Tkachuk ZY

2'-5'-linked triadenylates present the class of low molecular compounds, capable of regulating various cell functions. Their main function is the activation of ribonuclease L – the main enzyme of the innate immunity. Their dephosphorylation results in loss of this function, which, on the other hand grants them a handful of new, yet undiscovered abilities.
Aim: Study the concentration-dependend influence of dephosphorylated 2'-5'-linked tradenylates on the protein kinases activity.
Methods: Protein kinases were titrated with radioactively labeled ATP. After that the radioactivity value was determined with the use of scintillation counter.
Results: We have found out that 2'-5'-linked are capable of changing the biological activity of various protein kinases by altering the amount of ATP they can potentially cleave.
Conclusions: We suggest that this effect could occur to the specific changes in protein kinases structure.