Журнал продуктов питания: микробиология, безопасность и гигиена
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ISSN: 2476-2059
ISSN: 2476-2059
Adoui Faïza, Benyahia-Krid Férial Aziza, Boughellout Halima, Aissaoui-Zitoun Ouarda, Charrad Norhane, Haddouche Maroua and Zidoune Mohammed Nasereddine
The present work was carried out in order to study the antimicrobial activity of A. herba-alba extracts and their application as food preservative. A crude extract was prepared by steeping of the dry leaves of A. herba-alba in phosphate buffer. And, in order to obtain an enriched fraction in active molecules, a series of ammonium sulfate precipitation was carried out. Extracts showed antibacterial activity against E. feacalis, M. leteus and L. monocytogenes strains. However, the best activity is noted for the precipitate obtained at 60% of salt (ASP60), with zones of inhibition of the order of 23.67 ± 0.44 mm for the E. feacalis and M. leteus strains and 18.00 ± 0.67 mm for L. monocytogenes. This extract shows a MIC of 0.23 and 0.9 mg/ml for E. feacalis and L. monocytogenes, respectively. The application of ASP60 on traditional fresh cheese "Takammrite”, as preservative, cause a significant slowdown in microbial growth under refrigeration during 15 days of storage.