Анатомия и физиология: текущие исследования
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Autologous Platelet Rich Plasma (Platelet Gel): An Appropriate Intervention for Salvaging Cardiac Myocytes under Oxidative Stress after Myocardial Infarction

Barbara Y. Hargrave

Background: The prompt restoration of blood flow (reperfusion) to the ischemic myocardium after an acute myocardial infarction is critical to the survival of non damaged heart tissue. However, reperfusion is responsible for additional myocardial damage. Our objective was to investigate the role of autologous platelet rich plasma or platelet gel prepared using nanosecond pulsed electric fields (nsPEFs) in improving left ventricular mechanical function after ischemic reperfusion.

Methods: The hearts of nine adult female New Zealand White rabbits were perfused using the Langendorff model. The hearts were exposed to global ischemia for 30 min and reperfused for 60 min. The hearts were injected with 600 μl of platelet gel supernatant prepared using nsPEF or platelet gel supernatant prepared using bovine thrombin or with bovine serum albumin (BSA) which served as our control. HUV-EC cells or H9c2 were used in vitro to determine the effect of platelet gel on ROS formation and mitochondrial depolarization using flow cytometry. Metalloproteinase’s and their inhibitors were assessed using western blot analysis. HPLC was used to identify the presence of endogenously secreted antioxidants in the platelet gel.

Results: Platelet gel improved left ventricular mechanical function of the heart, reduced ROS formation and reduced mitochondrial depolarization. Platelet gel also decreased MMP-2 and increased TIMP-1. Catalase and superoxide dismutase were preserved at greater concentrations in the platelet gel made using nsPEFs than in the platelet gel made with bovine thrombin.

Conclusion: Platelet gel is cardioprotective to non- ischemic reperfused cardiac tissue after acute myocardial infarction and reperfusion.