Журнал исследований рака и иммуноонкологии
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Computational Analysis of Crrna to Regulate Hsa-Mir-301b-3p and Hsa-Mir-21 Related to Metastasis and Cell Proliferation in Acute Myeloid Leukemia (AML) Using CRISPR/C2c2

Omid Moeini*, Mahsa Khatibi, Seyed Armit Hosseini, AmirAli Rahmani, Mohammadmatin Nourikhani, Arshia Aliyarzadeh

Background: Myeloid or lymphoid progenitors in the bone marrow can develop into leukemia, which is clonal cancer. Acute Myeloid Leukemia (AML) developed as a result of somatic mutations in the precursor cells of the myeloid lineage along with transcriptome dysregulation of bone marrow infiltration, which resulted in the generation of immature myeloid cells (blasts) and disruption of normal hematopoiesis. Current research has demonstrated the metastasis potential of a certain subset of microRNAs. As a result, miRNA downregulation at the transcriptional level can reduce the possibility of metastasis. The purpose of this work is to analyze miRNA precursor targeting utilizing the CRISPR-C2c2 (Cas13a) method.

Results: crRNAs designed for miR-301b and miR-21 has a very high structural similarity with binding energy to the state observed in the normal condition.

Conclusion: Sequence based evaluation of crRNAs intended for RNA level editing is insufficient; simulation and molecular docking investigations should also be carried out for improved accuracy.