Журнал лекарственного метаболизма и токсикологии
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In vitro Effects of Salicylic Acid on Plasma Paraoxonase 1 Activity

Dhouha Haj Mouhamed, Asma Ezzaher, Lotfi Gaha, Wahiba Douki and Mohamed Fadhel Najjar

Objectives: Our study aimed to examine the effects of salicylic acid on paraoxonase activity in healthy volunteers. Methods: Plasma from healthy volunteers was spiked with salicylic acid drug. The working solutions were then diluted with plasma to obtain concentrations that covered the therapeutic margin. Specimens were incubated at 25 and 37°C, and we measured PON1 activities in the presence of different drug concentrations after 1 h, 3 h, and 24 h and after one week. Results: After incubation in vitro, a significant increase of paraoxonase 1 activity according to salicylic acid concentration and incubation duration, regardless of temperature incubation was showed. This increase was marked especially after 24 h of incubation. The stimulatory effect of salicylic acid is concentration and incubation durationdependent and does not seem to be related to temperature incubation. Conclusion: The tested Salicylic acid significantly stimulated PON1 activity in a concentration dependent manner. This result shows that acetyl salicylic acid treatments have a beneficial effect dose and time dependent that may contribute to prevent atherosclerosis.