Gang Yang, Yue Wang, Zhi Cui, Baoxia Due, Guoxiang Yin, Yang Yu, Wei Zhang
Objective: To study the expression of erythropoietin receptor (EPOR) after radiation injury of the submandibular gland in rats, and to provide theoretical basis for further exploring the dysfunction of salivary glands.
Methods: 30 Wistar rats were divided into 2 groups: the control group and the experimental group. A large dose of 15Gy radiation was used to irradiate the head and neck in rats, the control group did not receive radiation. Paraffin sections were made from the submandibular glands of rats in the control and experimental groups on the third days, thirtieth days and sixtieth days, respectively. HE staining, the expression of erythropoietin receptor protein was observed by immunohistochemical staining. The average optical density values were calculated according to Imagepro plus 6.0 for quantitative analysis.
Results: 1) The expression of EPOR in the submandibular glands of rats in the experimental group increased significantly after radiation, and was higher than the control group at the same time. 2) The expression of EPOR in the control group increased with age. 3) EPOR was found to be expressed in the cytoplasm and cytoplasm of glandular ducts, but not to be found in acinar cells.
Conclusion: The expression of EPOR increased after radiation injury of salivary glands in rats.