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Isolation, Screening and Optimized Production of Extracellular Xylanase under Submerged Condition from Aspergillus Flavus Mtcc 9390

Bharat Bhushan, Ajay Pal and Veena Jain

In order to isolate xylanolytic microbial strains, screening and isolation was done using agricultural waste and decaying biomass. The enzyme super-secreter Aspergillus flavus MTCC 9390 was selected for optimized production of xylanase. Various process variables were optimized using conventional ‘one-variable-at-a-time’ approach which involves varying a single independent variable and maintaining others at a constant level. All culture conditional variables had profound influence on enzyme production and 15-30% increase was brought by nitrogen source only. A synergistic five-fold increase in xylanase production was achieved when an inoculums size of 2 x 106 spores/ mL was incubated in modified Czapek Dox-A for 6 days at pH 6.0 and temperature 45ºC under static conditions in submerged fermentation.