Журнал хроматографии и методов разделения
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Liquid Chromatography

Belissa E, Nino C, Bernard M, Henriet T, Sadou-Yaye H, Surget E, Boccadifuoco G, Yagoubi N and Do B

Acute bacterial conjunctivitis and their complications are commonly treated by 6 mg/mL ticarcillin and 50 mg/mL vancomycin eyes drops, formulated separately in hospitals. A rapid, selective and specific LC-tandem MS method has been developed and validated for simultaneous quantification of ticarcillin and vancomycinin solution and in presence of degradation products. Atlantis® C18 Column T3 (250×4.6 mm I.D., 5 μm) and gradient mobile phase composed of 44 mM trifluoracetic acid/methanol allowed for good separation. Degradation products were separated on LC and detected with mass spectrometry in full scan mode. The method was suitable forthe stability study of ticarcillin-vancomycin pH 5.2 solutionsover an 8-day period storage at 5 ± 3°C and at room temperature (25 ± 2°C). The solutions remained stable up to 5 days at 5 ± 3°C. At room temperature, degradation of ticarcillin was accelerated in presence of vancomycin, while vancomycin stability was not further affected by ticarcillin. High-resolution mass spectrometry was used for the characterization of the degradation products.