Журнал гликобиологии
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Partial Purification, Characterization and Investigation of the Anti-Bacterial Activity of Trilepisium madagascariense Seed Lectin

Ilemobayo Victor Fasogbon*, Micheal Nosano Yakubu, Adam Mustapha, Chidiebere Obi, Nuhu Tanko, Danjuma Ali Zenoh, Mari Batta Mshelia, Aliyu Salihu, Ismaila Alhaji Umar, Muhammad Sani Aliyu

Published data on the properties and possible applications of the Lectin(s) from Trilepisium madagascariense seed is still limited. This study aimed to partially characterize and investigate the seed extract of T. madagascariense for antibacterial activity against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, using agar well diffusion method. The extract was partially purified by Sephadex G-75 filtration and characterized, to obtain a partially purified Trilepisium madagascariense Seed Lectin (ppTMSL).The ppTMSL was found to agglutinate all human erythrocytes (blood groups A, B, and O), showing no blood group specificity; although there was statistical increase (P<0.05) in its activity on blood group O erythrocytes while comparing the natural logarithm of the mean of specific activities of the TMSL with the other blood groups erythrocytes. ppTMSL was however found to betteragglutinate rabbit erythrocyte. Molecular weight estimation suggests a heterodimer of 22 kDa and 24 kDa respectively in the ppTMSL, having 7-8 and 25°C-50°C as the optimum pH and temperature for its maximum hemagglutinating activity. ppTMSL was found to lack sensitivity against all the bacterial isolate tested. The results will provide useful guidelines for further research on TMSL and its possible applications.