Журнал протеомики и биоинформатики
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Evolutionary Trace Analysis of Azoreductase at the Ligand Binding Site and Enhancing the Active Site through Site Directed Mutagenesis

Shobana S, Berla Thangam E and Kasmir Raja SV

Azoreductases belong to the family of oxidoreductases and are characterized with detoxification of azo dyes. Azoreductases are involved in bioremediation, to eliminate the color effluents found in waste waters. In this study, the effectiveness of azoreductase in bioremediation was analysed, and the aim was to find a way to increase the efficiency of the enzyme towards toxic compound degradation, more rapidly. To estimate this, the structure function relationship was determined by evolutionary trace (ET) analysis at ligand binding site. The class specific site of ET analysis was mapped with the crystal structure of azoreductase (1NNI). Site directed mutagenesis was performed at the residues, in very close proximity to the active site residues, hydrogen bonded to ligand. Based on the work done, it was found that Glycine at 106th position plays a crucial role in enzyme activity.