Журнал протеомики и биоинформатики
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Isolation, Cloning, DNA Sequencing and Bioinformatics Analysis of the Parasporin–1 Gene of Bacillus thuringiensis

Abdulrahman S Assaeedi and Gamal H Osman

Native strains of Bacillus thuringiensis were isolated and screened for the presence of parasporin–1 gene. Polymerase Chain Reaction (PCR) based DNA amplification using the gene-specific primers was used for the purpose. Of all the isolated strains, only four exhibited amplicons of a size analogous to that of the known parasporin–1 gene. The amplified fragments were cloned into the pGEM-vector and then were sequenced and analyzed. Bioinformatics analysis revealed that the nucleotide sequences obtained from the isolates were 99% homologous to the known DNA sequence of the parasporin gene (Blast software) and 98% homologous to the known amino acid sequence. The parasporin gene sequence obtained from this study was submitted to GenBank (Accession no. KJ576792). The 2371 nucleotides long gene was found to encode for a protein composed of 789 amino acids that had an estimated molecular weight of 84 kDa and a calculated isoelectric point (pI) of 6.7.