Передовые методы в биологии и медицине
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Large-Scale Cultures and Bioreactors for the Production of Megakaryocytes and Platelets

Anja Baigger, Dorothee Eicke, Rainer Blasczyk, Constanca Figueiredo

A constant rising demand on platelets has been observed which is associated with population ageing and rapid progresses in the development of advanced therapeutic strategies. Therefore, innovative attempts towards the in vitro production of platelets and their precursors are inevitable. Extensive research has been performed on the exploration of reliable cell sources and the establishment of efficient protocols for the differentiation of functional platelets. In particular, the design of bioreactors mimicking the bone marrow microenvironment has gained plenty of attention to achieve clinically relevant platelet yields. In this review, we summarize major advances and perspectives on the manufacture and application of in vitro generated megakaryocytes and platelets.