Журнал пробиотиков и здоровья
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Rejuvenating the Gut of Antibiotic Incited Zebrafish (Danio Rerio) by Probiotic Soil Isolates Persuaded by Subtilisin as Endogenous Protease Inhibitors

Arockiya Anita Margret1*, S Aishwarya2, A Shree Parkavi1, V Swetha1, T Suvedha1, G Sandhya1

Antibiotics are extensively used as therapeutics against various human and veterinary ailments. It is also used to promote livestock growth, which can be hazardous by its dispersion in water bodies and lead to aquatic toxicity. Antibiotic-associated diarrhea (AAD) is a common complication caused due to the adverse effects of antibiotic administration. It is necessary to have a better understanding on consumption of probiotics, possessing natural endogenous protease inhibitors and its mechanism to target protease from gut pathogens. Subtilisin are group of proteases originated from Bacillus spp and a molecular docking study revealed its inhibitory potential (-1.90 KJ/Mol) against a target protein Clostridium difficile toxin. The bacterial isolates from the vegetative soil were identified as Aneurinibacillus migulanus and assayed for probiotic efficiency. The production of subtilisin from the distinct soil isolate was quantified (0.961 mg/ml and the enzymatic activity with a yield of 241.1 U/ml) and detected by HPLC studies. The probiotic efficacy of the isolates and the validation of subtilisin as enhancers were authenticated on antibiotic incited zebra fish models. The LC50 and histopathological studies attested both acute toxicity and restoration level of extracted protein. Hence this study validates the soil isolates as probiotics and subtilisin as endogenous protease inhibitors