Журнал хроматографии и методов разделения
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Supercritical-Fluid Chromatography with Diode-Array Detection for Emerging Contaminants Determination in Water Samples. Method Validation and Estimation of the Uncertainty

Vilma del C Salvatierra-Stamp, Norma S Pano-Farias, Silvia G Ceballos-Magaña, Jorge Gonzalez, Valentin Ibarra-Galván and Roberto Muñiz-Valencia

Here we present a communication about the article “Salvatierra-Stamp VC, Ceballos-Magaña SG, Gonzalez J, Ibarra-Galván V, Muñiz-Valencia R (2015) Analytical method development for the determination of emerging contaminants in water using supercritical-fluid chromatography coupled with diode-array detection. Analytical and Bioanalytical Chemistry 407:4219-4226”. In this paper, a selective, linear, accurate and precise supercritical-fluid chromatography coupled with diode-array detection method was developed and validated for the determination of seven emerging contaminants: two pharmaceuticals, three endocrine disruptors, one bactericide and one pesticide. The compounds were base-line separated in around 10 minutes. Also, the method involved a sample treatment optimization by means of C18-OH solid phase extraction cartridges. The developed method was validated. In this sense, the correlation coefficient and recovery was higher than 0.9997 and 94%, respectively. Limit of detection and quantification was in the range of 0.10-1.59 μg/L and 0.31-4.83 μg/L, respectively. The measurement uncertainty was evaluated using the top-down model considering six sources of uncertainty. For all compounds, the uncertainty associated with accuracy and linearity regression was the main contribution to the combined uncertainty. Expanded uncertainties for each compound in method analysis were lower than 10.8%. Finally the method was successfully applied to environmental water samples.