Журнал науки и исследований рака
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The Key proteins as potential effective biomarkers under Intraoperative Radiotherapy (IORT) in tumor bed of Breast Cancer patients

Minoo Shahani1,2, Jafar Shakeri1 , Ali Tafti1 , Farid Moradian1 , Majid Samsami1 , Pantea Rohollah3 , Atieh Akbari1 and Mohammad Esmaeil Akbari1 *

Background: Radiotherapy (RT) is recommended to all patients undergoing Breast Conserving Surgery (BCS). Two strategies can be applied to irradiation, External Beam RT (EBRT) in addition, Intraoperative Radiation Therapy (IORT). The aim of this study was to introduce a protein biomarker panel related to molecular function under IORT.

Methods: Six Breast Cancer (BC) patients as a pilot study were treated by 12 Gy (Boost dose) and 21 Gy (Radical dose). Samples tissue included Margin before IORT (MB), and Margin 24 hours After IORT (MA24 h). Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) was performed to study proteomic of IORT-treated tumor bed.

Results: We classified 110 differentially expressed proteins (DEPs) as a protein biomarker panel by mapping the anno- tated coding region sequences to the reference canonical pathways in the KEGG database.

Conclusion: Our findings indicate that the DEPs may be key proteins in IORT-treated tumor bed and may serve as potential Effective biomarkers under IORT.