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The Changes of the Levels of Tumor Necrosis Factor α, Interferon γ and Vascular Endothelial Growth Factor in the Sera of Children with Acquired Aplastic Anemia after Blood Transfusion

Li Yuyun, Li Ying, Zhang Haixia, Yan Guangxing, Xu Feng, Jia Zhiyi, Wang Fengyu, Zhang Yu, Xue Yuanyuan, Wang Lingzi, Chao Chunxia, Ma Liji, Zhang Xiaoyue and Bi Fangjie

Objective: To discuss how blood transfusion affects the progression of acquired aplastic anemia by assaying the levels of TNF-α, IFN-γ and VEGF in the sera of children with acquired aplastic anemia both before and after blood transfusion

Methods: In the study, the sera of 15 children with acquired aplastic anemia were collected both before and one or two days after blood transfusion. And during the study period the sera of 12 normal children who had body checkups in our hospital were also collected. The levels of TNF-α, IFN-γ and VEGF in the sera of children were assayed by the method of ELISA with the corresponding kits.

Results: The levels of TNF-α and IFN-γ in the sera of children with acquired aplastic anemia were 2.29 ± 0.36 ng/L and 21.30 ± 9.12 ng/L respectively. After blood transfusion they were 1.27 ± 0.12 ng/L and 15.33 ± 4.45 ng/L respectively. The levels of TNF-α and IFN-γ in the sera of children with acquired aplastic anemia after blood transfusion were lower than these before blood transfusion. But they were higher than those in the sera of normal children (TNF-α 0.85 ± 0.04 ng/L, IFN-γ 10.91 ± 4.67 ng/L) respectively. The level of VEGF in the sera of patients with acquired aplastic anemia was 50.07 ± 12.83 Pg/L and it was 80.02 ± 11.04 Pg/L after blood transfusion. The level of VEGF in the sera of patients with acquired aplastic anemia after blood transfusion was higher than this before blood transfusion, and both of them were lower than that in the sera of normal children (100.77 ± 36.83 Pg/L). There were significant differences between them (p<0.05).

Conclusions: Blood transfusion can decrease the levels of TNF-α and IFN-γ and increase the level of VEGF in the sera of children with acquired aplastic anemia, which may affect the progression of the disease.